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myd88 signaling regulation cells  (Addgene inc)


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    Addgene inc myd88 signaling regulation cells
    Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
    Myd88 Signaling Regulation Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myd88+signaling+regulation+cells/MYD88+(Plasmid+%2313092)/pm39550582-82-7-22
    Average 93 stars, based on 18 article reviews
    myd88 signaling regulation cells - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis."

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.

    Journal: Cell communication and signaling : CCS

    doi: 10.1186/s12964-024-01930-1

    Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
    Figure Legend Snippet: Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated

    Techniques Used: Mutagenesis, Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Injection, Transformation Assay

    Related Articles

    Transfection:

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [19], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 104 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http:// n2t. net/ addge ne: 13092; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [ ], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 10 4 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88 wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http://n2t.net/addgene:13092 ; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88 D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.

    Plasmid Preparation:

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [19], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 104 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http:// n2t. net/ addge ne: 13092; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [ ], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 10 4 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88 wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http://n2t.net/addgene:13092 ; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88 D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.

    Luciferase:

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [19], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 104 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http:// n2t. net/ addge ne: 13092; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis
    Article Snippet: HEK293 MyD88 KO cells, prepared in our lab [ ], were growing in DMEM containing 10% FBS (both Invitrogen) and 6 × 10 4 cells/96-well were seeded. .. To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88 wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http://n2t.net/addgene:13092 ; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88 D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen). .. 6 h after transfection, media was changed and cells were stimulated with 10 ng/ml of recombinant human IL-1β (Invivogen) for 16 h and afterwards lysed.



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    myd88 signaling regulation cells  (Addgene inc)
    93
    Addgene inc myd88 signaling regulation cells
    Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and <t>MyD88</t> mRNA expression was detected using qPCR in colon tissue homogenate. G <t>MyD88</t> <t>protein</t> detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated
    Myd88 Signaling Regulation Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myd88+signaling+regulation+cells/MYD88+(Plasmid+%2313092)/pm39550582-82-7-22
    Average 93 stars, based on 1 article reviews
    myd88 signaling regulation cells - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated

    Journal: Cell communication and signaling : CCS

    Article Title: MyD88 protein destabilization mitigates NF-κB-dependent protection against macrophage apoptosis.

    doi: 10.1186/s12964-024-01930-1

    Figure Lengend Snippet: Fig. 1 MyD88D162E mutant mice are protected from DSS-induced acute colitis. Schematic presentation of the DSS-induced colitis experiment. To induce acute colitis, mice were drinking tap water, supplemented with 2% DSS for 8 consecutive days ad libitum. A Disease activity index was defined by observing the stool consistency and the presence of the gross or occult blood. The weight loss was calculated from the weight difference on day1 and day 8 and the colon length was measured from the body of the caecum to the distal part of the rectum. B The part of the colon was washed and IL-6 cytokine was measured using ELISA assay. C Blood was collected and IL-6 was detected in serum using ELISA. D-F Il6, Il1b, and MyD88 mRNA expression was detected using qPCR in colon tissue homogenate. G MyD88 protein detection from obtained colon tissue of treated mice, using Western blot. β-actin was used as loading control. H Schematic presentation of the experiment. LPS was i.p. injected. After 4 h IL-6 and IL-10 cytokines were analyzed in the serum. Combined data from one (B, C. H) or two (A, D-F) indep. exp. are shown as mean ± SEM. LN transformation was applied for the data used in statistical tests (D-F). Studentʾs unpaired t-test was used. p values of < 0.05 (*), < 0.01 (**) are indicated

    Article Snippet: To determine the role of caspase-1 in MyD88 signaling regulation cells were transfected with pcDNA3.1 mouse MyD88wt (a gift from R. Medzhitov (Addgene plasmid #13,092; http:// n2t. net/ addge ne: 13092; RRID:Addgene_13092)) or pcDNA3.1 mouse MyD88D162E plasmids (both 0.05 ng/well) w/o increasing concentrations of pCMV mouse Caspase-1 (0.05, 0.1 ng/well) as well as pELAM1-luciferase (NF-κB promotor; 30 ng/well)) (a gift from C. Kirschning, Germany) and phRL-TK Renilla luciferase (3 ng/well) (Promega) for normalization using lipofectamine 2000 transfection reagent (Invitrogen).

    Techniques: Mutagenesis, Activity Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control, Injection, Transformation Assay